Part:BBa_K2332020

LuxCDABE (E. coli bioluminescence)

This part encodes the production of the proteins required for bacterial luminescence including luciferase and its substrates from Vibrio fischeri, codon optimised for E. coli. Naturally, Vibrio fischeri use additional regulatory proteins (encoded by LuxR and LuxI upstream) whose external concentration increases as a function of increasing cell-population density. Bacteria detect the accumulation of a minimal threshold stimulatory concentration of these autoinducers and regulate the expression of LuxCDABE. Consequently, we removed these regulatory proteins to ensure that cell concentration dependent luminescence through quorum sensing is surpassed and our cultures can fluoresce independently.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2562
  Illegal AgeI site found at 2829
  Illegal AgeI site found at 3357
  Illegal AgeI site found at 3938
  Illegal AgeI site found at 4385
  Illegal AgeI site found at 5263
  Illegal AgeI site found at 5478
  
• 1000
  COMPATIBLE WITH RFC[1000]